Adipose tissue-derived stromal cells (ASCs) are a promising candidates for cellular therapy in the field of regenerative medicine. … The short procedure time allows for intraoperative isolation of 1 mL of stromal vascular fraction derived from 10 mL of centrifuged adipose tissue.
How do you isolate an SVF?
The most widely used technique for the isolation of SVF from lipoaspirate is by digestion of the fatty portion of the lipoaspirate with collagenase, separating the contents into two distinct phases: the floating mature adipocytes fraction, and the cellular components of interest in the lower aqueous fraction [17, 28].
What is the stromal vascular fraction?
Stromal Vascular Fraction (SVF) is a heterogeneous collection of cells contained within adipose tissue that is traditionally isolated using enzymes such as collagenase.
How do you find the stromal vascular fraction?
SVF cells are obtained through liposuction and contain multiple cell types, including adipose-derived stem cells (ADSCs), mesenchymal and endothelial progenitor cells, leukocyte subtypes, lymphatic cells, pericytes, and vascular smooth muscle cells.
How do you collect adipose tissue?
Adipose collection occurs under local anesthesia with an abdominal biopsy that collects about two grams of fat. The fat is used to isolate and grow mesenchymal stem cells. These function of these cells will be tested for their therapeutic potential.
Can bone marrow be injected?
We have recently developed an innovative bone marrow transplantation (BMT) method, intra-bone marrow (IBM)-BMT, in which donor bone marrow cells (BMCs) are injected directly into the recipient bone marrow (BM), resulting in the rapid recovery of donor hemopoiesis and permitting a reduction in radiation doses as a …
How long does stem cell therapy last?
The stem cells injected into the target area will continue working for up to one year, and many patients do not require multiple treatments. Consider the benefits of trying stem cell therapy for your joint problems and experience an effective alternative to surgery.
What is Adipose?
Adipose tissue is commonly known as body fat. It is found all over the body. It can be found under the skin (subcutaneous fat), packed around internal organs (visceral fat), between muscles, within bone marrow and in breast tissue. … However, women tend to store more subcutaneous fat within the buttocks and thighs.